{
"cells": [
{
"cell_type": "markdown",
"id": "32087d19-f65e-465b-ab2b-f9c78420a61d",
"metadata": {},
"source": [
"# Divide by Gaussian\n",
"\n",
"When processing images with membrane staining it sometimes happens that the intensity of the membranes is not homogeneous and changes locally. This intensity variation may impair cell segmentation algorithms. In these cases it may make sense to homogenize the intensity by dividing the image by a Gaussian blurred version of itself."
]
},
{
"cell_type": "code",
"execution_count": 1,
"id": "142cfb4f-63b8-438c-a18f-348af9aa1ec0",
"metadata": {},
"outputs": [],
"source": [
"import pyclesperanto_prototype as cle\n",
"from skimage.io import imread, imshow\n",
"from skimage.filters import gaussian"
]
},
{
"cell_type": "markdown",
"id": "13658c3a-0ce6-4edd-8d22-2359c3074520",
"metadata": {},
"source": [
"In this image you see that the intensity of the membranes decreases from top to bottom."
]
},
{
"cell_type": "code",
"execution_count": 2,
"id": "313b3b95-6d47-42d0-a60a-88e6604d3fd6",
"metadata": {},
"outputs": [
{
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